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Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography

Författare:
Publiceringsår: 2008
Språk: Engelska
Sidor: 240-247
Publikation/Tidskrift/Serie: Journal of Microscopy
Volym: 232
Nummer: 2
Dokumenttyp: Artikel
Förlag: Wiley InterScience / Royal Microscopical Society

Sammanfattning

Manual cell counting is time consuming and requires a high
degree of skill on behalf of the person performing the count.
Here we use a technique that utilizes digital holography,
allowing label-free and completely non-invasive cell counting
directly in cell culture vessels with adherent viable cells.
The images produced can provide both quantitative and
qualitative phase information from a single hologram.
The recently constructed microscope HolomonitorTM (Phase
Holographic Imaging AB, Lund, Sweden) combines the
commonly used phase contrast microscope with digital
holography, the latter giving us the possibility of achieving
quantitative information on cellular shape, area, confluence
and optical thickness. This project aimed at determining the
accuracy and repeatability of cell counting measurements
using digital holography compared to the conventional
manual cell counting method using a haemocytometer. The
collected datawere also used to determine cell size and cellular
optical thickness.Theresults showthat digital holography can
be used for non-invasive automatic cell counting as precisely
as conventional manual cell counting

Disputation

Nyckelord

  • Biology and Life Sciences
  • Medicine and Health Sciences
  • Technology and Engineering
  • Cell analysis
  • cellular optical thickness
  • cell size
  • cell counting
  • digital holography

Övriga

Published
Yes
  • Electromagnetic theory
  • ISSN: 1365-2818 (online)
  • ISSN: 0022-2720 (print)

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