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Quaternary Structure Buildt from Subunuts Combining NMR and Small-Angle X-Ray Scattering Data

In English

Författare

  • M-L Mattinen
  • K Pääkkönen
  • T Ikonen
  • J Craven
  • Torbjörn Drakenberg
  • R Serimaa
  • J Waltho
  • A Annila

Summary, in English

A new principle in constructing molecular complexes from the known high-resolution domain structures joining data from NMR and small-angle x-ray scattering (SAXS) measurements is described. Structure of calmodulin in complex with trifluoperazine was built from N- and C-terminal domains oriented based on residual dipolar couplings measured by NMR in a dilute liquid crystal, and the overall shape of the complex was derived from SAXS data. The residual dipolar coupling data serves to reduce angular degrees of freedom, and the small-angle scattering data serves to confine the translational degrees of freedom. The complex built by this method was found to be consistent with the known crystal structure. The study demonstrates how approximate tertiary structures of modular proteins or quaternary structures composed of subunits can be assembled from high-resolution structures of domains or subunits using mutually complementary NMR and SAXS data.

Avdelning/ar

Publiceringsår

2002

Språk

Engelska

Sidor

1177-1183

Publikation/Tidskrift/Serie

Biophysical Journal

Volym

83

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Cell Press

Ämne

  • Biophysics

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1542-0086