Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

Summary, in English

A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.

• Författare: Ibolya Bontidean; Josefin Ahlqvist; Ashok Mulchandani; Wilfred Chen; Weon Bae; Rajesh K Mehra; Alessia Mortari; Elisabeth Csöregi
• Avdelning/ar: Kemiska institutionen; Bioteknik
• Publiceringsår: 2003
• Språk: Engelska
• Sidor: 547-553
• Publikation/Tidskrift/Serie: Biosensors & Bioelectronics
• Volym: 18
• Issue: 5-6
• Länkar: http://dx.doi.org/10.1016/S0956-5663(03)00026-5
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Industrial Biotechnology
• Nyckelord: Biosensors; Heavy metals; Phytochelatins; Capacitance
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1873-4235