Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique
Författare
Summary, in English
An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.
Avdelning/ar
Publiceringsår
1986
Språk
Engelska
Sidor
1399-1403
Publikation/Tidskrift/Serie
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volym
34
Issue
11
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Histochemical Society
Ämne
- Medical and Health Sciences
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1551-5044