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Detection of Helicobacter ganmani-Like 16S rDNA in Pediatric Liver Tissue

In English

Författare

  • Vasundhara Tolia
  • Hans-Olof Nilsson
  • Kimberly Boyer
  • Anne Wuerth
  • Waleed Abu Al-Soud
  • Raja Rabah
  • Torkel Wadström

Summary, in English

Background. To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis.Materials and methods. Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects with no evidence of preexisting liver disease were examined for the presence of Helicobacter species by a genus-specific seminested polymerase chain reaction (PCR) assay. PCRproducts of positive samples were further characterized by denaturing gradient gel electrophoresis (DGGE) and DNA-sequence analysis. Based on those results, a seminested PCR assay for H. ganmani was developed and applied to the samples.Results. On analysis, 40/61 patient samples were positive in the genus-specific Helicobacter PCR and 4/10 from the control group. The nucleotide sequences of 16S rDNA fragments were 99100 similar to mainly Helicobacter sp. liver and H. ganmani. PCR-products similar to H. canis and H. bilis were also found. The 16S rDNAs of control specimens showed similarity to Helicobacter sp. liver. In the H. ganmani-specific PCR analysis 19 patients, but none of the controls, were positive.Conclusions. Amplified Helicobacter 16S rDNAs were related to Helicobacter sp. liver or H. ganmani in liver biopsy specimens of pediatric patients. The possible significance of Helicobacter species in pediatric liver diseases needs to be evaluated further in prospective studies.

Publiceringsår

2004

Språk

Engelska

Sidor

460-468

Publikation/Tidskrift/Serie

Helicobacter

Volym

9

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Microbiology in the medical area

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1083-4389