Du är här

Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Författare:
Publiceringsår: 2003
Språk: Engelska
Sidor: 457-463
Publikation/Tidskrift/Serie: Canadian Journal of Botany
Volym: 81
Nummer: 5
Dokumenttyp: Artikel
Förlag: NRC Press

Sammanfattning

Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a single, radial intercellular hypha. These "paired" arbuscules often appeared to be at different developmental stages. Logistic regression and measurement of surface area indicated that there was a delay in initiation of the second arbuscule.

Disputation

Nyckelord

  • Biology and Life Sciences

Övriga

Published
Yes
  • Microbial Ecology
  • ISSN: 1480-3305

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

 

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen