Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Endotoxin Exposure In Sewage Treatment Workers: Investigation Of Exposure Variability And Comparison Of Analytical Techniques

In English

Författare

  • Suzanne Spaan
  • Lidwien A. M. Smit
  • Wijnand Eduard
  • Lennart Larsson
  • Huib J. J. M. Arts
  • Inge M. Wouters
  • Dick J. J. Heederik

Summary, in English

Introduction: Objectives were to give an overview of endotoxin exposure and its determinants in sewage treatment workers, and to study exposure to culturable and non-culturable microorganisms and the applicability of the LAL assay in this work environment. Material and methods: In 43 Dutch sewage treatment plants 470 full-shift, 123 task-based personal and 54 stationary inhalable dust samples were collected. Endotoxin concentration was determined with the LAL-assay. Mixed effects models were used to investigate possible determinants of exposure. Simultaneous parallel filter Samples, impinger samples and viable total bacteria and Gram-negative bacterial samples were taken to compare analytical techniques. Filter and impinger samples were analyzed with the LAL-assay, gas chromatography-mass spectrometry (GC-MS) and fluorescence microscopy. Results: Endotoxin exposure levels were moderate to low (geometric mean personal exposure 27 EU/m(3), stationary 33 EU/m(3), task-based 64 EU/m(3)), yet differences between jobs and sources and some determinants of exposure were identified. Exposure varied more from day to day than between workers. Concentrations in filter samples were higher and more consistent than in impinger samples. Fungi and Gram-positive bacteria were found in higher levels than Gram-negative bacteria. The LAL assay and GC-MS showed comparable endotoxin levels. Discussion and conclusion: Endotoxin exposure in Dutch sewage treatment workers was relatively low,. Comparison of sampling and analytical techniques suggests that the LAL-assay did not result in much exposure misclassification. It thus seems justified to perform filter measurements in combination with the LAL-assay to measure endotoxin exposure in sewage treatment plants.

Publiceringsår

2008

Språk

Engelska

Sidor

251-261

Publikation/Tidskrift/Serie

Annals of Agricultural and Environmental Medicine

Volym

15

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Institute of Agricultural Medicine in Lublin, Poland

Ämne

  • Microbiology in the medical area

Nyckelord

  • sewage
  • non-culturable microorganisms
  • culturable and
  • analyzing techniques
  • endotoxin
  • exposure variability

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1898-2263