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Clinical isolates of Streptococcus pneumoniae bind the complement inhibitor C4b-binding protein in a PspC allele-dependent fashion.

In English

Författare

Summary, in English

The complement system constitutes an important component of the innate immune system. To colonize their host and/or to cause disease, many pathogens have evolved strategies to avoid complement-mediated bacterial lysis and opsonophagocytosis. In this study, using a collection of 55 clinical isolates of Streptococcus pneumoniae, we demonstrate for the first time that pneumococci bind the complement inhibitor C4b-binding protein (C4BP). C4BP binding seems to be restricted to certain serotypes such as serotype 4, 6B, 7F, and 14, of which the strains of serotype 14 are the strongest binders. We show that bacteria-bound C4BP retains its functional activity and down-regulates the activation of the classical pathway. Thus, this major respiratory pathogen may escape immune recognition and eradication by the complement system. Furthermore, we show that C4BP binding varies between strains but is dependent on the expression of pneumococcal surface protein C, PspC of group 4. The study of the distribution of group 4 pspC locus shows that most of high-binder serotype 14 isolates harbor an allelic variant of group 4 pspC. Using PspC-negative mutant strains, we identified a new allelic variant of PspC (PspC4.4) as a major ligand for C4BP, revealing a new function for this important pneumococcal virulence factor. Thus pneumococci exploit host C4BP for complement evasion in a PspC allele-dependent manner.

Publiceringsår

2009

Språk

Engelska

Sidor

7865-7877

Publikation/Tidskrift/Serie

Journal of Immunology

Volym

182

Issue

12

Dokumenttyp

Artikel i tidskrift

Förlag

American Association of Immunologists

Ämne

  • Immunology in the medical area

Nyckelord

  • Bacterial Proteins: metabolism
  • Bacterial Proteins: genetics
  • Bacterial Proteins: immunology
  • Complement C4b-Binding Protein: genetics
  • Complement C4b-Binding Protein: immunology
  • Complement C4b-Binding Protein: metabolism
  • Mutation: genetics
  • Streptococcus pneumoniae: genetics
  • Streptococcus pneumoniae: immunology
  • Streptococcus pneumoniae: metabolism
  • Streptococcus pneumoniae: isolation & purification

Status

Published

Forskningsgrupp

  • Protein Chemistry, Malmö
  • Clinical Microbiology, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 1550-6606