Tissue repair in lung disorders
Författare
Summary, in English
The remodeling phase of tissue repair in lung disorders such as idiopathic pulmonary disease, asthma, obliterative bronchiolitis, and after lung transplantation is not well understood. One of the key players in fibrosis is the fibroblast and its progenitor, the fibrocyte. The fibroblast is the main producer of extracellular matrix molecules such as collagen, versican, perlecan, biglycan, and decorin. Fibrocytes are recruited from the bone marrow to the site of injury. It is possible that the stromal cell-derived factor-1/CXCL12 builds up in a gradient to recruit CXCR4 expressing fibrocytes, at least in the case of idiopathic pulmonary fibrosis. Patients with obliterative bronchilitis have an enlarged vessel lumen and a larger endothelial layer area, which has been shown to be correlated to more fibrocytes in the tissue. A correlation was found between the numbers of fibrocytes identified in tissue and structural changes in the lung. In idiopathic pulmonary fibrosis, a correlation was found between numbers of fibrocytes and numbers of fibroblastic foci, and in patients with obliterative bronchiolitis there was a correlation between fibrocyte numbers and thickening of the alveolar parenchyma. Eventually the fibrocyte enters the tissue and can differentiate into a fibroblast.
Matrix production by fibroblasts in patients who have undergone lung transplantation and in asthmatic subjects involves both the central and the distal parts of the lung. In lung-transplanted patients, the production of proteoglycans in the distal part was found to be double that in the central part. Versican production was particularly elevated compared to controls. The production of proteoglycans was further correlated with proliferation rate. A common feature of fibroblasts in patients after lung transplantation and asthmatic subjects was that these cells proliferated more slowly than in control subjects. Distally-derived fibroblasts from asthmatic subjects produced more veriscan, were immobile, and had many protrusions. The distal parts of the lungs were also rich in collagen.
A better understanding of fibrocyte and fibroblast function will help us to characterise the mechanisms behind idiopathic pulmonary disease, asthma, obliterative bronchiolitis. This knowledge can then be applied to other types of fibrotic disorders.
Matrix production by fibroblasts in patients who have undergone lung transplantation and in asthmatic subjects involves both the central and the distal parts of the lung. In lung-transplanted patients, the production of proteoglycans in the distal part was found to be double that in the central part. Versican production was particularly elevated compared to controls. The production of proteoglycans was further correlated with proliferation rate. A common feature of fibroblasts in patients after lung transplantation and asthmatic subjects was that these cells proliferated more slowly than in control subjects. Distally-derived fibroblasts from asthmatic subjects produced more veriscan, were immobile, and had many protrusions. The distal parts of the lungs were also rich in collagen.
A better understanding of fibrocyte and fibroblast function will help us to characterise the mechanisms behind idiopathic pulmonary disease, asthma, obliterative bronchiolitis. This knowledge can then be applied to other types of fibrotic disorders.
Publiceringsår
2009
Språk
Engelska
Publikation/Tidskrift/Serie
Lund University Faculty of Medicine Doctoral Dissertation Series
Volym
2009:122
Fulltext
Dokumenttyp
Doktorsavhandling
Förlag
Lund University: Faculty of Medicine
Ämne
- Respiratory Medicine and Allergy
- Cell and Molecular Biology
Status
Published
Forskningsgrupp
- Lung Biology
ISBN/ISSN/Övrigt
- ISSN: 1652-8220
- ISBN: 978-91-86443-11-5
Försvarsdatum
11 december 2009
Försvarstid
09:00
Försvarsplats
Belfragesalen, D15, BMC, Lund
Opponent
- Gunnar Pejler