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Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases

Publiceringsår: 2004
Språk: Engelska
Sidor: 3769-3777
Publikation/Tidskrift/Serie: Electrophoresis
Volym: 25
Nummer: 21-22
Dokumenttyp: Artikel
Förlag: WILEY-VCH Verlag GmbH & Co.

Sammanfattning

The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.

Disputation

Nyckelord

  • Chemistry

Övriga

Published
Yes
  • ISSN: 0173-0835

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