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Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells

Författare:
  • Claire Hebrard
  • Emeline Cros-Perrial
  • Anders Ranegaard Clausen
  • Charles Dumontet
  • Jure Piskur (Professor)
  • Lars Petter Jordheim
Publiceringsår: 2009
Språk: Engelska
Sidor: 1068-1075
Publikation/Tidskrift/Serie: Nucleosides Nucleotides & Nucleic Acids
Volym: 28
Nummer: 11-12
Dokumenttyp: Artikel
Förlag: Taylor & Francis Inc

Sammanfattning

Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.

Disputation

Nyckelord

  • Biology and Life Sciences
  • bacteria
  • suicide gene
  • gene therapy
  • Deoxyribonucleoside kinases
  • gemcitabine
  • cancer
  • resistance

Övriga

Published
Yes
  • ISSN: 1525-7770

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