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Quantification of Membrane Proteins Using Nonspecific Protease Digestions

Författare:
Publiceringsår: 2009
Språk: Engelska
Sidor: 5666-5673
Publikation/Tidskrift/Serie: Journal of Proteome Research
Volym: 8
Nummer: 12
Dokumenttyp: Artikel
Förlag: Amer Chemical Soc

Sammanfattning

We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • membrane proteins
  • N-terminal labeling
  • quantification
  • relative
  • proteinase K
  • Bacillus subtilis

Övriga

Published
Yes
  • ISSN: 1535-3893

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