Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Helicobacter species DNA in liver and gastric tissues in children and adolescents with chronic liver disease

In English

Författare

  • Thomas H. Casswall
  • Antal Nemeth
  • Ingrid Nilsson
  • Torkel Wadström
  • Hans-Olof Nilsson

Summary, in English

Objective. Enterohepatic Helicobacter species (EHS) have previously been found in adults with hepatobiliary diseases. Here, we report the prevalence of Helicobacter pylori and EHS in liver and gastric tissue in children and adolescents with chronic liver disease (CLD). Material and methods. Seventy-seven consecutive children and adolescents with CLD with or without ulcerative colitis or Crohn's disease (UC/CD) were investigated. Tissue samples were analysed using a Helicobacter genus specific 16S rDNA polymerase chain reaction (PCR) assay and DNA-sequence analysis. Sera from 61 subjects were also analysed using enzyme immunoassay and immunoblotting. Results. The Helicobacter PCR was positive in 3/23 (13%) livers from patients with primary sclerosing cholangitis and UC, and in 1/2 livers from patients with autoimmune hepatitis (AIH) and UC. Sequenced PCR products matched the 16S rDNA of H. hepaticus, H. muridarum, H. canis, and H. pylori, respectively. H. ganmani and H. bilis were detected in gastric tissues from two AIH patients. H. hepaticus and H. pullorum were found in livers from two patients with acute liver failure and intrahepatic cholestasis. Antibody reactivity to Helicobacter cell-surface proteins was negative. Conclusions. H. pylori and EHS can be detected in the livers of some patients with UC and concomitant liver disease, as well as in other children with liver diseases. Multicentre studies from different locations are needed to find out whether these bacteria play a pathogenetic role or whether their presence is an epiphenomenon.

Publiceringsår

2010

Språk

Engelska

Sidor

160-167

Publikation/Tidskrift/Serie

Scandinavian Journal of Gastroenterology

Volym

45

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Taylor & Francis

Ämne

  • Gastroenterology and Hepatology

Nyckelord

  • colitis
  • ulcerative
  • primary sclerosing cholangitis
  • polymerase chain reaction
  • liver tissue
  • inflammatory bowel disease
  • Helicobacter pylori
  • enterohepatic Helicobacter species
  • Autoimmune hepatitis
  • children

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1502-7708