Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor

In English

Författare

Summary, in English

A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

Publiceringsår

2005

Språk

Engelska

Sidor

983-990

Publikation/Tidskrift/Serie

Biotechnology Letters

Volym

27

Issue

14

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Industrial Biotechnology
  • Control Engineering

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1573-6776