Meny

Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor

Författare:
Publiceringsår: 2005
Språk: Engelska
Sidor: 983-990
Publikation/Tidskrift/Serie: Biotechnology Letters
Volym: 27
Nummer: 14
Dokumenttyp: Artikel
Förlag: Springer

Sammanfattning

A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

Disputation

Nyckelord

  • Chemistry
  • Technology and Engineering

Övriga

Published
Yes
  • ISSN: 0141-5492

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen