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Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens

In English

Författare

  • Tomas Garcia-Caballero
  • Dorthe Grabau
  • Andrew R. Green
  • John Gregory
  • Arno Schad
  • Elke Kohlwes
  • Ian O. Ellis
  • Sarah Watts
  • Jens Mollerup

Summary, in English

Aims: Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. Methods and results: We found 100% agreement between HER2 status determined by FISH and dual-colour CISH. Furthermore, we observed that the time used to score slides was significantly reduced by 28% in dual-colour CISH compared with the FISH protocol. Concordance between HER2 protein status and dual-colour CISH or FISH was equally good with an overall agreement of 96.8%. Correlation between the HER2/centromere 17 gene ratios obtained with dual-colour CISH and FISH was highly significant with an overall correlation coefficient (rho) of 0.96. Conclusions: We conclude that dual-colour CISH and bright field microscopy are excellent alternatives to FISH when analysing the HER2 status of primary breast cancer.

Avdelning/ar

Publiceringsår

2010

Språk

Engelska

Sidor

472-480

Publikation/Tidskrift/Serie

Histopathology

Volym

56

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Cell and Molecular Biology

Nyckelord

  • hybridization
  • in situ
  • HER2 amplification
  • HER2
  • FISH
  • CISH
  • breast cancer
  • CEN-17

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0309-0167