The IgD C(H)1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID.

Summary, in English

The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody structure. When amino acids 157-224 of the IgD C(H)1 region were substituted with IgG sequences, binding by M. catarrhalis or recombinant MID962-1200 was abolished. Subsequent smaller substitutions of IgD C(H)1 157-224 with IgG sequences led us to conclude that IgD C(H)1 amino acids 198-206 were crucial for the interaction between MID and IgD.

• Författare: Martin Samuelsson; Johan Jendholm; Stefan Amisten; Sherie L Morrison; Arne Forsgren; Kristian Riesbeck
• Avdelning/ar: Klinisk mikrobiologi, Malmö; Islet cell physiology
• Publiceringsår: 2006
• Språk: Engelska
• Sidor: 2525-2534
• Publikation/Tidskrift/Serie: European Journal of Immunology
• Volym: 9
• Issue: Sep;36
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16906531&dopt=Abstract; http://dx.doi.org/10.1002/eji.200535594
• Dokumenttyp: Artikel i tidskrift
• Förlag: John Wiley & Sons Inc.
• Ämne: Immunology in the medical area
• Nyckelord: Moraxella catarrhalis; immunoglobulin D; binding
• Status: Published
• Forskningsgrupp: Clinical Microbiology, Malmö; Islet cell physiology
• ISBN/ISSN/Övrigt: ISSN: 1521-4141