Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).

Summary, in English

Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.

• Författare: Martin Samuelsson; Arne Forsgren; Kristian Riesbeck
• Avdelning/ar: Klinisk mikrobiologi, Malmö
• Publiceringsår: 2006
• Språk: Engelska
• Sidor: 31-37
• Publikation/Tidskrift/Serie: Journal of Immunological Methods
• Volym: 317
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17056056&dopt=Abstract; http://dx.doi.org/10.1016/j.jim.2006.09.007
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Immunology in the medical area
• Nyckelord: purification; immunoglobulin D; Moraxella catarrhalis; MID
• Status: Published
• Forskningsgrupp: Clinical Microbiology, Malmö
• ISBN/ISSN/Övrigt: ISSN: 1872-7905