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Non-contact acoustic cell trapping in disposable glass capillaries.

Författare:
Publiceringsår: 2010
Språk: Engelska
Sidor: 2251-2257
Publikation/Tidskrift/Serie: Lab on a chip
Volym: 10
Dokumenttyp: Artikel
Förlag: Royal Society of Chemistry

Sammanfattning

Non-contact trapping using acoustic standing waves has shown promising results in cell-based research lately. However, the devices demonstrated are normally fabricated using microfabrication or precision machining methods leading to a high unit cost. In e.g. clinical or forensic applications avoiding cross-contamination, carryover or infection is of outmost importance. In these applications disposable devices are key elements, thus making the cost per unit a critical factor. A solution is presented here where low-cost off-the-shelf glass capillaries are used as resonators for standing wave trapping. Single-mode as well as multi-node trapping is demonstrated with an excellent agreement between simulated and experimentally found operation frequencies. Single particle trapping is verified at 7.53 MHz with a trapping force on a 10 mum particle of up to 1.27 nN. The non-contact trapping is proved using confocal microscopy. Finally, an application is presented where the capillary is used as a pipette for aspirating, trapping and dispensing red blood cells.

Disputation

Nyckelord

  • Technology and Engineering

Övrigt

Published
Yes
  • ISSN: 1473-0197

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