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Neofunctionalization in an ancestral insect desaturase lineage led to rare Delta(6) pheromone signals in the Chinese tussah silkworm

In English

Författare

Summary, in English

The Chinese tussah silkworm, Antheraea pernyi (Lepidoptera: Saturniidae) produces a rare dienoic sex pheromone composed of (E,Z)-6,11-hexadecadienal, (E,Z)-6,11-hexadecadienyl acetate and (E,Z)-4,9-tetradecadienyl acetate, and for which the biosynthetic routes are yet unresolved. By means of gland composition analyses and in vivo labeling we evidenced that pheromone biosynthesis towards the immediate dienoic gland precursor, the (E,Z)-6,11-hexadecadienoic acid, involves desaturation steps with Delta(6) and Delta(11) regioselectivity. cDNA cloning of pheromone gland desaturases and heterologous expression in yeast demonstrated that the 6,11-dienoic pheromone is generated from two biosynthetic routes implicating a Delta(6) and Delta(11) desaturase duo albeit with an inverted reaction order. The two desaturases first catalyze the formation of the (E)-6-hexadecenoic acid or (Z)-11-hexadecenoic acid, key mono-unsaturated biosynthetic intermediates. Subsequently, each enzyme is able to produce the (E,Z)-6,11-hexadecadienoic acid by accommodating its non-respective mono-unsaturated product. Besides elucidating an unusually flexible pheromone biosynthetic pathway, our data provide the first identification of a biosynthetic Delta(6) desaturase involved in insect mate communication. The occurrence of this novel Delta(6) desaturase function is consistent with an evolutionary scenario involving neo-functionalization of an ancestral desaturase belonging to a gene lineage different from the Delta11-desaturases commonly involved in moth pheromone biosynthesis.

Publiceringsår

2010

Språk

Engelska

Sidor

742-751

Publikation/Tidskrift/Serie

Insect Biochemistry and Molecular Biology

Volym

40

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Zoology
  • Biological Sciences

Status

Published

Projekt

  • The pheromone brewery
  • Evolutionary mechanisms of pheromone divergence in Lepidoptera

Forskningsgrupp

  • Pheromone Group

ISBN/ISSN/Övrigt

  • ISSN: 1879-0240