Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.

Summary, in English

AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.

• Författare: Peren H Karagin; Unne Stenram; Torkel Wadström; Åsa Ljungh
• Avdelning/ar: Tumörmikromiljö; Avdelningen för medicinsk mikrobiologi
• Publiceringsår: 2010
• Språk: Engelska
• Sidor: 4817-4822
• Publikation/Tidskrift/Serie: World Journal of Gastroenterology
• Volym: 16
• Issue: 38
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/20939110?dopt=Abstract; http://dx.doi.org/10.3748/wjg.v16.i38.4817
• Dokumenttyp: Artikel i tidskrift
• Förlag: WJG Press
• Ämne: Gastroenterology and Hepatology
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1007-9327