Meny

Javascript is not activated in your browser. This website needs javascript activated to work properly.
Du är här

Dosage Compensation by Gene-Copy Silencing in a Triploid Hybrid Fish

Författare:
Publiceringsår: 2008
Språk: Engelska
Sidor: 1344-1348
Publikation/Tidskrift/Serie: Current biology : CB
Volym: 18
Nummer: 17
Dokumenttyp: Artikel
Förlag: Elsevier Ltd.

Sammanfattning

In mammals, the increase in gene dosage, in the form of polyploidy or involving chromosomal fragments, has deleterious effects [1]. Regulation of appropriate gene product amounts has to be warranted by complex dosage-compensation mechanisms. Lower vertebrates, on the other hand, cope very well with ploidy increase [2 4], implying either effective compensation or a lack of necessity for such mechanisms. Unfortunately, nothing is known about the genetic and molecular mechanisms underlying this phenomenon. For an experimental approach, we have studied gene expression in the allotriploid form of Squalius alburnoides. In these organisms, different genomes are joined through hybridization; thus, sequence differences can be used to follow expression of different alleles [5, 6]. We found that a compensation mechanism exists, reducing transcript levels to the diploid state. Our data suggest a silencing of one of the three alleles. Unexpectedly, it is not a whole haplome that is inactivated. The allelic expression patterns differ between genes and between different tissues for one and the same gene. Our data provide the first evidence of a regulation mechanism involving gene-copy silencing in a triploid vertebrate.

Disputation

Nyckelord

  • Biology and Life Sciences
  • DEVBIO

Övriga

Published
Yes
  • ISSN: 0960-9822

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen