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The effects of caldesmon extraction on mechanical properties of skinned smooth muscle fibre preparations

In English

Författare

Summary, in English

The role of caldesmon in the regulation of smooth muscle contraction was investigated in chemically skinned smooth muscle fibres from the guinea-pig taenia coli. A 19-kDa C-terminal fragment of caldesmon gave a minor (<5%) reduction of force in fully thiophosphorylated fibres, but reduced force by about 50% at intermediate activation levels without affecting the level of light chain phosphorylation. An extraction procedure was developed using incubation in solutions containing high Mg2+ concentrations. Protein analysis revealed a selective decrease in the amount of caldesmon in the fibres. Maximal active force per cross-sectional area was unaffected. The Ca2+ dependence of active force was shifted towards lower Ca2+ concentrations and became less steep. The effects of extraction of caldesmon could in part be reversed by incubation in a solution containing purified caldesmon. The results are consistent with the hypothesis that caldesmon in smooth muscle thin filaments inhibits force generation and plays a role in regulating cooperative attachment of cross-bridges at sub-maximal levels of activation in smooth muscle.

Publiceringsår

1996

Språk

Engelska

Sidor

241-247

Publikation/Tidskrift/Serie

Pflügers Archiv

Volym

432

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Pharmacology and Toxicology
  • Medicinal Chemistry

Nyckelord

  • Skinned smooth muscle
  • Caldesmon
  • 19-kDa fragment
  • Calcium sensitivity

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0031-6768