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Antiproliferative Activity of Melanoidins Isolated from Heated Potato Fiber (Potex) in Glioma Cell Culture Model

Författare:
  • Ewa Langner
  • Fernando M. Nunes
  • Piotr Pozarowski
  • Martyna Kandefer-Szerszen
  • Stefan Pierzynowski
  • Wojciech Rzeski
Publiceringsår: 2011
Språk: Engelska
Sidor: 2708-2716
Publikation/Tidskrift/Serie: Journal of Agricultural and Food Chemistry
Volym: 59
Nummer: 6
Dokumenttyp: Artikel
Förlag: Amer Chemical Soc

Sammanfattning

Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 degrees C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with low molecular weight fraction. Impaired MAPK (mitogen-activated protein kinase) and Akt signaling was found in cells treated with the extract. Moreover, flow cytometry analyses revealed the extract to induce G(1)/S arrest in glioma cells. Simultaneously, Western blot analysis showed elevated levels of p21 protein with concomitant decrease of cyclin D1. In conclusion, observed antiproliferative activity of melanoidins present in heated Potex was linked to disregulated MAPK and Akt signaling pathways, as well as to cell cycle cessation. These results suggest potential application of Potex preparation as a functional food ingredient and chemopreventive agent.

Disputation

Nyckelord

  • Biology and Life Sciences
  • antiproliferative effect
  • cell cycle
  • melanoidins
  • potato fiber
  • protein kinase signaling

Övriga

Published
Yes
  • ISSN: 0021-8561

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