Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm

Summary, in English

We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.

• Författare: Anna Tranell; Susanne Tingsborg; Eva Maria Fenyö; Stefan Schwartz
• Avdelning/ar: Avdelningen för medicinsk mikrobiologi
• Publiceringsår: 2011
• Språk: Engelska
• Sidor: 82-91
• Publikation/Tidskrift/Serie: Virus Research
• Volym: 157
• Issue: 1
• Länkar: http://dx.doi.org/10.1016/j.virusres.2011.02.010
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Microbiology in the medical area
• Nyckelord: HIV-1; vpr; SRp55; Splicing
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1872-7492