Hormonemate, a new cytotoxic and apoptosis-inducing compound from the endophytic fungus Hormonema dematioides. I. Identification of the producing strain, and isolation and biological properties of hormonemate
Författare
Summary, in English
In a search for new fungal compounds inducing apoptosis of the colon cancer derived cell line COLO-320, hormonemate (1) was purified from fermentations of an endophytic fungus isolated from living needles of a Pinus species. The producing strain was identified as Hormonema dematioides by microscopy and ITS rDNA sequence analysis. The structure of hormonemate was determined by spectroscopic methods. The compound exhibited cytotoxic effects against the human colon tumor cell lines COLO-320, DLD-1 and HT-29 as well as five other human cell lines (HL-60, JURKAT, HEP-G2, MCF-7, HeLa S3). It also induced apoptosis in COLO-320 cells as detected by a caspase-activity assay and morphological changes, and it triggered morphological and physiological differentiation of HL-60 cells into granulocytes, which subsequently died by apoptosis.
Avdelning/ar
Publiceringsår
2003
Språk
Engelska
Sidor
547-552
Publikation/Tidskrift/Serie
Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences
Volym
58
Issue
7-8
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Verlag der Zeitschrift für Naturforschung
Ämne
- Organic Chemistry
Nyckelord
- hormonemate
- endophyte
- Hormonema dematioides
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0939-5075