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Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics

Författare:
  • P Akerblad
  • Robert Månsson
  • Anna Lagergren
  • S Westerlund
  • B Basta
  • U Lind
  • A Thelin
  • Ramiro Gisler
  • David Liberg
  • S Nelander
  • K Bamberg
  • Mikael Sigvardsson
Publiceringsår: 2005
Språk: Engelska
Sidor: 206-216
Publikation/Tidskrift/Serie: Physiological Genomics
Volym: 23
Nummer: 2
Dokumenttyp: Artikel
Förlag: American Physiological Society

Sammanfattning

Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.

Disputation

Nyckelord

  • Chemistry
  • Medicine and Health Sciences
  • early B cell factor
  • peroxisome proliferator-activated
  • adipogenesis
  • receptor-gamma 2

Övriga

Published
Yes
  • ISSN: 1094-8341

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