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The dual regulator Sufu integrates Hedgehog and Wnt signals in the early Xenopus embryo

Författare:
  • Hooi Min Tan Grahn
  • Martin Kriebel
  • Shirui Hou
  • Edgar Pera
Publiceringsår: 2011
Språk: Engelska
Sidor: 262-276
Publikation/Tidskrift/Serie: Developmental Biology
Volym: 358
Nummer: 1
Dokumenttyp: Artikel
Förlag: Academic Press Inc Elsevier Science

Sammanfattning

Hedgehog (Hh) and Wnt proteins are important signals implicated in several aspects of embryonic development, including the early development of the central nervous system. We found that Xenopus Suppressor-of-fused (XSufu) affects neural induction and patterning by regulating the Hh/Gli and Wnt/beta-catenin pathways. Microinjection of XSufu mRNA induced expansion of the epidermis at the expense of neural plate tissue and caused enlargement of the eyes. An antisense morpholino oligonucleotide against XSufu had the opposite effect. Interestingly, both gain- and loss-of-function experiments resulted in a posterior shift of brain markers, suggesting a biphasic effect of XSufu on anteroposterior patterning. XSufu blocked early Wnt/beta-catenin signaling, as indicated by the suppression of XWnt8-induced secondary axis formation in mRNA-injected embryos, and activation of Wnt target genes in XSufu-MO-injected ectodermal explants. We show that XSufu binds to XG*i1 and X beta-catenin. In Xenopus embryos and mouse embryonic fibroblasts, Gli1 inhibits Wnt signaling under overexpression of beta-catenin, whereas beta-catenin stimulates Hh signaling under overexpression of Glil. Notably, endogenous Sufu is critically involved in this crosstalk The results suggest that XSufu may act as a common regulator of Hh and Wnt signaling and contribute to intertwining the two pathways. (C) 2011 Elsevier Inc. All rights reserved.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • Suppressor-of-fused
  • Gli
  • beta-Catenin
  • Neural
  • Xenopus

Övrigt

Published
Yes
  • ISSN: 0012-1606

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