Meny

Javascript is not activated in your browser. This website needs javascript activated to work properly.
Du är här

Metabolism of [N-15]alanine in the ectomycorrhizal fungus Paxillus involutus

Författare:
Publiceringsår: 1995
Språk: Engelska
Sidor: 297-304
Publikation/Tidskrift/Serie: Experimental Mycology
Volym: 19
Nummer: 4
Dokumenttyp: Artikel
Förlag: Elsevier B.V / Academic Press

Sammanfattning

Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [N-15]alanine. Short-term exposure of mycelial discs to [N-15]alanine showed that the greatest flow of N-15 was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions, resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [N-15]alanine rapidly accumulated. This evidence indicates the direct involvement of alanine aminotransferase for translocation of N-15 from alanine to glutamate. Alanine may be a convenient reservoir of both nitrogen and carbon. (C) 1995 Academic Press, Inc.

Disputation

Nyckelord

  • Biology and Life Sciences
  • alanine metabolism
  • alanine aminotransferase
  • aminooxyacetate
  • ectomycorrhizal fungus
  • organic nitrogen utilization
  • Paxillus involutus

Övriga

Published
Yes
  • Microbial Ecology
  • ISSN: 0140-962X (Print)

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen