Isolation of fluorescein diacetate stained hyphae from soil by micromanipulation

In English Stained fluorescent hyphae were picked out from soil using a Skerman micromanipulator. About 30% of the plated out hyphae produced visible colonies, at least half of which could be considered to be derived from hyphae, The dominating genera were Penicillium, Oidiodendron and Mortierella. Non-sporulating fungi were rather few (4'5%). Results were
compared with those obtained using the dilution plate and soil washing techniques.