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The quantitative proteome of a human cell line

Författare:
  • Martin Beck
  • Alexander Schmidt
  • Johan Malmström
  • Manfred Claassen
  • Alessandro Ori
  • Anna Szymborska
  • Franz Herzog
  • Oliver Rinner
  • Jan Ellenberg
  • Ruedi Aebersold
Publiceringsår: 2011
Språk: Engelska
Publikation/Tidskrift/Serie: Molecular Systems Biology
Volym: 7
Dokumenttyp: Artikel
Förlag: Nature Publishing Group

Sammanfattning

The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least similar to 10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution. Molecular Systems Biology 7: 549; published online 8 November 2011; doi:10.1038/msb.2011.82

Disputation

Nyckelord

  • Medicine and Health Sciences
  • mass spectrometry
  • protein abundance
  • proteomics

Övriga

Published
Yes
  • ISSN: 1744-4292

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