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The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

Författare:
Publiceringsår: 2001
Språk: Engelska
Sidor: 409-416
Publikation/Tidskrift/Serie: Combinatorial Chemistry & High Throughput Screening
Volym: 4
Nummer: 5
Dokumenttyp: Artikel

Sammanfattning

Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • COMPLEMENTARITY-DETERMINING REGIONS
  • PHAGE DISPLAY LIBRARY
  • SINGLE-CHAIN
  • FV
  • AFFINITY HUMAN-ANTIBODIES
  • BY-PASSING IMMUNIZATION
  • RANDOM
  • MUTAGENESIS
  • BINDING-SITE
  • V-H
  • SYNTHETIC REPERTOIRES
  • SOMATIC
  • HYPERMUTATION

Övriga

Published
Yes
  • ISSN: 1386-2073

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