A gene expression fingerprint of mouse stomach ECL cells
Författare
Summary, in English
Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in all attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.
Avdelning/ar
- Drug Target Discovery
Publiceringsår
2005
Språk
Engelska
Sidor
404-410
Publikation/Tidskrift/Serie
Biochemical and Biophysical Research Communications
Volym
332
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- enterochromaffin-like cells
- microarray
- inbred C57BL/6
- mice
- stomach
- gastric mucosa
- ECL cells
- parathyroid hormone-like hormone
- parathyroid hormone-related peptide
Status
Published
Forskningsgrupp
- Drug Target Discovery
ISBN/ISSN/Övrigt
- ISSN: 1090-2104