Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro

Summary, in English

Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.

• Författare: Emma S Anderson; Carl Bjartmar; Cecilia Eriksson Linsmeier; Gunilla Westermark; Claes Hildebrand
• Publiceringsår: 2000
• Språk: Engelska
• Sidor: 21-24
• Publikation/Tidskrift/Serie: Neuroscience Letters
• Volym: 284
• Issue: 1-2
• Länkar: http://dx.doi.org/10.1016/S0304-3940(00)00989-7
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Neurosciences
• Nyckelord: Cell culture; T4-O; Myelination; Development; White matter; Spinal cord; Oligodendrocyte; Chicken
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0304-3940