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Comparative cytogenetic and DNA flow cytometric analysis of 242 primary breast carcinomas

In English

Författare

Summary, in English

The cytogenetic and DNA flow cytometric findings in 242 breast carcinomas were compared. The combined use of both techniques improved the detection of abnormal cell populations from 65% by cytogenetic analysis alone and 59% by DNA flow cytometric analysis alone to 84%. Informative and comparable cytogenetic and flow cytometric data were obtained for 155 tumors. Among these 155 tumors, there was good concordance (64%) between the estimates of genomic changes by the two methods. Most discrepancies were among the DNA-diploid cases, where cytogenetic analysis detected small genomic changes. There were, however, also some exceptions in which large genomic changes detected by one method were missed by the other. Of the specific breast cancer-associated cytogenetic aberrations subjected to separate correlation analysis, polysomy for chromosome 20 was significantly associated with a high S-phase fraction, whereas loss of the long arm of chromosome 16 and/or the presence of a der(1;16) were significantly associated with a low S-phase fraction. Our data show that cytogenetic and DNA flow cytometric analyses of breast carcinomas give largely comparable results, and that combining data from both methods significantly improves the information obtained by either technique used alone on the genetic abnormalities in these tumors.

Publiceringsår

2003-11

Språk

Engelska

Sidor

62-67

Publikation/Tidskrift/Serie

Cancer Genetics and Cytogenetics

Volym

147

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Medical Genetics
  • Cancer and Oncology

Nyckelord

  • Breast Neoplasms
  • Carcinoma
  • Chromosome Aberrations
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16
  • Chromosomes, Human, Pair 20
  • Cytogenetic Analysis
  • Diploidy
  • Female
  • Flow Cytometry
  • Humans
  • Karyotyping
  • Ploidies
  • S Phase
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

Status

Published

Forskningsgrupp

  • Lund Melanoma Study Group

ISBN/ISSN/Övrigt

  • ISSN: 0165-4608