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CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney

Författare:
Publiceringsår: 2009
Språk: Engelska
Sidor: 2038-2044
Publikation/Tidskrift/Serie: Nephrology Dialysis Transplantation
Volym: 24
Nummer: 7
Dokumenttyp: Artikel i tidskrift
Förlag: Oxford University Press

Sammanfattning

Background. CRIM1 is a plasma membrane bound protein

containing six cysteine-rich repeats (CRR). Through these,

CRIM1 has been shown to interact with a subgroup of the

TGF-β superfamily, the bone morphogenic proteins (BMP)

isoforms 2, 4 and 7. The probable action is to modulate

the signalling properties of these factors. CRIM1 has also

been shown to regulate the release of VEGFA by podocytes

during renal organogenesis. Knock-out studies in mice have

shown that CRIM1 is critically involved in the development

of the central nervous system, eye and kidney. Replacement

of CRIM1 with a defective version leads to renal dysgenesis

and perinatal death. We have analysed the distribution of

CRIM1 in adult human renal tissue.

Methods. To this end, we have used immunofluorescence,

immunohistochemistry and immunoelectron microscopy.

We performed western blotting for the CRIM1 protein,

using lysates from isolated glomerular podocytes and human

renal tissue homogenate. By using quantitative PCR,

we compared the CRIM1 mRNA levels in podocytes, human

renal tissue homogenate, primary human renal proximal

tubular epithelial cells and primary human pulmonary

artery smooth muscle cells.

Results. The results show that in the human adult kidney,

CRIM1 is mainly expressed in the glomerular podocytes

and is associated with the insertional region of the filtration

slit diaphragm (SD) of the podocyte pedicles.

Conclusions. CRIM1 is a protein that should be added to

the list of proteins associated with the podocyte filtration

SD and with the probable action of modulating BMP and

VEGFA signalling.

Nyckelord

  • Urology and Nephrology
  • podocyte
  • immunoelectron microscopy
  • bone morphogenic protein
  • filtration slit membrane

Övriga

Published
  • Pathology, Malmö
  • ISSN: 1460-2385

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