Moraxella-Dependent {alpha}1-Antichymotrypsin Neutralization - A Unique Virulence Mechanism.
Författare
Summary, in English
The acute phase reactant and protease inhibitor alpha1-antichymotrypsin is considered to play a protective role in the airways, but it is not known. Objectives: We analyzed whether the common respiratory pathogens Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis interact with antichymotrypsin. Methods: We compared a series of clinical isolates in addition to wild type and ubiquitous surface protein-deficient Moraxella to study the nature of antichymotrypsin binding by the bacteria. Measurements and Main Results: M. catarrhalis was the only species that bound antichymotrypsin among 25 bacterial species tested by flow cytometry and a direct binding assay. Experiments with Moraxella mutants revealed that ubiquitous surface proteins A1 and A2 were responsible for the interaction, and using recombinant fragments, a consensus sequence within ubiquitous surface proteins A1 and A2 was defined. Binding of iodine labeled antichymotrypsin was dose dependent and strong (d
Avdelning/ar
Publiceringsår
2008
Språk
Engelska
Sidor
609-617
Publikation/Tidskrift/Serie
American Journal of Respiratory Cell and Molecular Biology
Volym
38
Issue
5
Dokumenttyp
Artikel i tidskrift
Förlag
American Thoracic Society
Ämne
- Cell and Molecular Biology
Status
Published
Forskningsgrupp
- Clinical Microbiology, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1535-4989