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Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins.

In English

Författare

Summary, in English

Composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. The composites with immobilized concanavalin A (Con A) were used for capturing glycoproteins. Adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. The monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions at sub-zero temperature. The cryogels were epoxy activated and Con A was immobilized as an affinity ligand. Binding and elution of horseradish peroxidase (HRP) was studied in batch experiment and in a chromatographic setup. Increasing adsorbent concentration in composite cryogels will increase ligand density, which therefore enhances the amount of bound HRP from 0.98 till 2.9 (milligram enzyme per milliliter of gel) in the chromatographic system. The material was evaluated in 10 cycles for binding and elution of HRP.

Avdelning/ar

Publiceringsår

2012

Språk

Engelska

Sidor

2978-2985

Publikation/Tidskrift/Serie

Journal of Separation Science

Volym

35

Issue

21

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Industrial Biotechnology

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1615-9314