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Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems.

Publiceringsår: 2012
Språk: Engelska
Sidor: 4296-4304
Publikation/Tidskrift/Serie: Lab on a chip
Volym: 12
Nummer: 21
Dokumenttyp: Artikel
Förlag: Royal Society of Chemistry

Sammanfattning

Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12 micrometer-sized particles in the acoustic trap prior to the sub-micron particle capture. Furthermore, the new seeded-particle-aided acoustic trapping technique was employed for the continuous enrichment of bacteria (E. coli) with a capture efficiency of 95%. Finally, seed particle assisted acoustic trapping and enrichment is demonstrated for polymer-based particles down to 110 nm in diameter.

Disputation

Nyckelord

  • Biology and Life Sciences

Övrigt

Published
Yes
  • ISSN: 1473-0189

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