Characterization of a novel testicular form of human hormone-sensitive lipase
Författare
Summary, in English
Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.
Publiceringsår
2002
Språk
Engelska
Sidor
286-290
Publikation/Tidskrift/Serie
Biochemical and Biophysical Research Communications
Volym
291
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- testis
- spermatogenesis
- lipid metabolism
- fertility
- lipase
- esterase
Status
Published
Forskningsgrupp
- Insulin Signal Transduction
- Molecular Endocrinology
ISBN/ISSN/Övrigt
- ISSN: 1090-2104