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Metagenomic sequencing of "HPV-negative" condylomas detects novel putative HPV types.

In English

Författare

  • Hanna K Johansson
  • Davit Bzhalava
  • Johanna Ekström
  • Emilie Hultin
  • Joakim Dillner
  • Ola Forslund

Summary, in English

Condylomas are caused by human papillomavirus (HPV), but may in rare cases be "negative for HPV" by PCR. Metagenomic sequencing can be used for an unbiased assessment of the presence of virus. Ten swab sample pools, each containing four cases of "HPV-negative" condylomas, were subjected to metagenomic sequencing. One pool contained Molluscum contagiosum. Five pools contained HPV, of which three pools contained novel putative HPV-types. The 12 samples in these three pools were sequenced individually. Six of these contained HPV and two contained Molluscum contagiosum. Altogether, 1337 HPV-related reads were detected, representing 23 novel putative Gammapapillomaviruses, 10 established HPV types (genital HPV types 6, 57, 58 and 66, Betapapillomavirus types 5, 105, 124, and Gammapapillomavirus types 50, 130, 150) and two described HPV sequences (KC7 and FA69). Complete genomes of Gammapillomavirus FA69 and SE87 were compiled. Metagenomic sequencing reveals that seemingly "HPV-negative" condylomas contain known and previously unknown HPV types.

Avdelning/ar

Publiceringsår

2013

Språk

Engelska

Sidor

1-7

Publikation/Tidskrift/Serie

Virology

Volym

440

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Microbiology in the medical area

Status

Published

Forskningsgrupp

  • Clinical Microbiology, Malmö
  • Internal Medicine - Epidemiology

ISBN/ISSN/Övrigt

  • ISSN: 1096-0341