Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species

Summary, in English

Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.

• Författare: Mattias Gustafsson; Colin N. Palmer; Roland C. Wolf; Claes von Wachenfeldt
• Avdelning/ar: Institutionen för laboratoriemedicin; Molekylär cellbiologi; Mikrobiologigruppen
• Publiceringsår: 2001
• Språk: Engelska
• Sidor: 459-464
• Publikation/Tidskrift/Serie: Archives of Microbiology
• Volym: 176
• Issue: 6
• Länkar: http://dx.doi.org/10.1007/s002030100350
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Immunology in the medical area; Microbiology in the medical area
• Nyckelord: Cyp102A2; Cyp102A1; FatR; Bacillus subtilis; cytochrome P450; P450BM-3; Cyp102A3
• Status: Published
• Forskningsgrupp: Microbiology Group
• ISBN/ISSN/Övrigt: ISSN: 0302-8933