The Lecticans of Mammalian Brain Perineural Net Are O-Mannosylated

Summary, in English

O-Mannosylation is an important protein modification in brain. During the last years, a few mammalian proteins have been identified as targets of the protein-O-mannosyltransferases 1 and 2. However, these still cannot explain the high content of O-mannosyl glycans in brain and the strong brain involvement of congenital muscular dystrophies caused by POMT mutations (Walker-Warburg syndrome, dystroglycanopathies). By fractionating and analyzing the glycoproteome of mouse and calf brain lysates, we could show that proteins of the perineural net, the lecticans, are O-mannosylated, indicating that major components of neuronal extracellular matrix are O-mannosylated in mammalian brain. This finding corresponds with the high content of O-mannosyl glycans in brain as well as with the brain involvement of dystroglycanopathies. In contrast, the lectican neurocan is not O-mannosylated when recombinantly expressed in EBNA-293 cells, revealing the possibility of different control mechanisms for the initiation of O-mannosylation in different cell types.

• Författare: Sandra Pacharra; Franz-Georg Hanisch; Martina Muehlenhoff; Andreas Faissner; Uwe Rauch; Isabelle Breloy
• Avdelning/ar: Kärlväggsbiologi
• Publiceringsår: 2013
• Språk: Engelska
• Sidor: 1764-1771
• Publikation/Tidskrift/Serie: Journal of Proteome Research
• Volym: 12
• Issue: 4
• Länkar: http://dx.doi.org/10.1021/pr3011028
• Dokumenttyp: Artikel i tidskrift
• Förlag: The American Chemical Society (ACS)
• Ämne: Cell and Molecular Biology
• Nyckelord: O-glycans; O-mannosylation; lecticans; ECM; perineural net; O-glycosylation; ESI-MS/MS; MALDI-MS/MS; glycoproteomics; dystroglycanopathies
• Status: Published
• Forskningsgrupp: Vessel Wall Biology
• ISBN/ISSN/Övrigt: ISSN: 1535-3893