Assignment of the human gene for beta-microseminoprotein (MSMB) to chromosome 10 and demonstration of related genes in other vertebrates
Författare
Summary, in English
The gene for beta-microseminoprotein MSMB has been studied by DNA hybridization and molecular cloning techniques. Comparative analysis of restriction endonuclease digests of the cloned gene and of leukocyte DNA strongly suggested that the gene is present in a single copy in the haploid human genome. By Southern blot analysis of DNA from somatic cell hybrids, the gene was assigned to chromosome 10. The coding nucleotides of the human gene are separated into four exons by relatively large introns. A related gene might be present in other mammals, birds, and amphibians as revealed by DNA hybridization under conditions of low stringency.
Avdelning/ar
Publiceringsår
1991
Språk
Engelska
Sidor
4-920
Publikation/Tidskrift/Serie
Genomics
Volym
11
Issue
4
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Academic Press
Ämne
- Medicinal Chemistry
Nyckelord
- *Prostatic Secretory Proteins
- Molecular Sequence Data
- Humans
- Dna
- Molecular
- Cloning
- Pair 10
- Human
- *Chromosomes
- Chromosome Mapping
- Southern
- Blotting
- Animals
- Base Sequence
- Proteins/*genetics
- Research Support
- Non-U.S. Gov't
- Restriction Mapping
- Semen/*metabolism
- Seminal Plasma Proteins
- Vertebrates/genetics
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1089-8646