Du är här

Affinity partitioning of a Cellulomonas fimi beta-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system

Författare:
Publiceringsår: 2006
Språk: Engelska
Sidor: 53-59
Publikation/Tidskrift/Serie: JOURNAL OF CHROMATOGRAPHY A
Volym: 1123
Nummer: 1
Dokumenttyp: Artikel
Förlag: ELSEVIER SCIENCE BV

Sammanfattning

A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination with the genetic fusion of any given protein to the mannan-binding module, the results envision a general procedure for primary affinity recovery of such fusion proteins.

Disputation

Nyckelord

  • Chemistry
  • galactomannan
  • affinity-partitioning
  • aqueous two-phase system
  • beta-mannanase
  • mannan-binding module

Övriga

Published
Yes
  • ISSN: 0021-9673

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

 

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen