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Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target

Publiceringsår: 2006
Språk: Engelska
Sidor: 1071-1081
Publikation/Tidskrift/Serie: Journal of Proteomic Research
Volym: 5
Nummer: 5
Dokumenttyp: Artikel
Förlag: American Chemical Society

Sammanfattning

A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.

Disputation

Nyckelord

  • Technology and Engineering
  • proteomics
  • spectrometry
  • mass
  • SPE
  • solid-phase extraction
  • solid-phase microextraction
  • SPME
  • integrated selective enrichment target
  • ISET
  • MALDI MS

Övriga

Published
Yes
  • ISSN: 1535-3893

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