Expression profiling of Wilms tumors reveals new candidate genes for different clinical parameters
Författare
Summary, in English
Wilms tumor is the most frequent renal neoplasm in children, but our understanding of its genetic basis is still limited. We performed cDNA microarray experiments using 63 primary Wilms tumors with the aim of detecting new candidate genes associated with malignancy grade and tumor progression. All tumors had received preoperative chemotherapy as mandated by the SIOP protocol, which sets this study apart from related approaches in the Unites States that are based on untreated samples. The stratification of expression data according to clinical criteria allowed a rather clear distinction between different subsets of Wilms tumors. Clear-cut differences in expression patterns were discovered between relapse-free as opposed to relapsed tumors and tumors with intermediate risk as opposed to high risk histology. Several differentially expressed genes, e.g. TRIM22, CENPF, MYCN, CTGF, RARRES3 and EZH2, were associated with Wilms tumor progression. For a subset of differentially expressed genes, microarray data were confirmed by real-time RT-PCR on the original set of tumors. Interestingly, we found the retinoic acid pathway to be deregulated at different levels in advanced tumors suggesting that treatment of these tumors with retinoic acid may represent a promising novel therapeutic approach. (c) 2005 Wiley-Liss, Inc.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
1954-1962
Publikation/Tidskrift/Serie
International Journal of Cancer
Volym
118
Issue
8
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Cancer and Oncology
Nyckelord
- E2F
- microarray
- retinoic acid
- Wilms tumor
- nephroblastoma
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0020-7136