Experimental Models to Study Drug Distributions in Tissue Using MALDI Mass Spectrometry
Författare
Summary, in English
Requirements for patient safety and improved efficacy are steadily increasing in modern healthcare and are key drivers in modern drug development. New drug characterization assays are central in providing evidence of the specificity and selectivity of drugs. Meeting this need, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is used to study drug localization within micro-environmental tissue compartments. Thin sections of human lung tumor and rat xenograft tissues were exposed to pharmaceutical drugs by either spotting or submerging. These drugs, the epidermal growth factor receptor antagonists, erlotinib (Tarceva™) and gefitinib (Iressa™), and the acetylcholine receptor antagonist, tiotropium were characterized by microenvironment localization. Intact tissue blocks were also immersed in drug solution followed by sectioning. MALDI-MSI was then performed using a Thermo MALDI LTQ Orbitrap XL instrument to localize drug distribution patterns. We propose three MALDI-MSI models measuring drug disposition that have been used to map the selected compounds within tissue compartments of tumors isolated from lung cancer patients.
Avdelning/ar
- Avdelningen för Biomedicinsk teknik
- Institutionen för kliniska vetenskaper, Lund
- Tumörmikromiljö
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publiceringsår
2013
Språk
Engelska
Sidor
5626-5633
Publikation/Tidskrift/Serie
Journal of Proteome Research
Volym
12
Issue
12
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Medical Engineering
Nyckelord
- Lung Cancer
- Adenocarcinoma
- MALDI-Mass Spectrometry Imaging
- Erlotinib
- Gefitinib
- Tiotropium
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1535-3893