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Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model

In English

Författare

  • Ira M. Sigar
  • Justin H. Schripsema
  • Yibing Wang
  • Ian N. Clarke
  • Lesley T. Cutcliffe
  • Helena M. B. Seth-Smith
  • Nicholas R. Thomson
  • Carina Bjartling
  • Magnus Unemo
  • Kenneth Persson
  • Kyle H. Ramsey
Visa allt

Summary, in English

We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.

Publiceringsår

2014

Språk

Engelska

Sidor

61-69

Publikation/Tidskrift/Serie

Pathogens and Disease

Volym

70

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Microbiology in the medical area

Nyckelord

  • Chlamydia
  • plasmid
  • mouse
  • virulence
  • infection
  • genome

Status

Published

Forskningsgrupp

  • Urogynaecology and Reproductive Pharmacology
  • Clinical Microbiology, Malmö

ISBN/ISSN/Övrigt

  • ISSN: 2049-632X