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Interactions of PAMAM Dendrimers with Negatively Charged Model Biomembranes.

In English

Författare

  • Marianna Yanez
  • Marie-Louise Ainalem
  • Lionel Porcar
  • Anne Martel
  • Helena Coker
  • Dan Lundberg
  • Debby P Chang
  • Olaf Soltwedel
  • Robert Barker
  • Tommy Nylander

Summary, in English

We have investigated the interactions between cationic poly(amidoamine) (PAMAM) dendrimers of generation 4 (G4), a potential gene transfection vector, with net-anionic model biomembranes composed of different ratios of zwitterionic phosphocholine (PC) and anionic phospho-l-serine (PS) phospholipids. Two types of model membranes were used: solid-supported bilayers, prepared with lipids carrying palmitoyl-oleoyl (PO) and diphytanoyl (DPh) acyl chains, and free-standing bilayers, formed at the interface between two aqueous droplets in oil (droplet interface bilayers, DIBs) using the DPh-based lipids. G4 dendrimers were found to translocate through POPC:POPS bilayers deposited on silica surfaces. The charge density of the bilayer affects translocation, which is reduced when the ionic strength increases. This shows that the dendrimer-bilayer interactions are largely controlled by their electrostatic attraction. The structure of the solid-supported bilayers remains intact upon translocation of the dendrimer. However, the amount of lipids in the bilayer decreases and dendrimer/lipid aggregates are formed in bulk solution, which can be deposited on the interfacial layers upon dilution of the system with dendrimer-free solvent. Electrophysiology measurements on DIBs confirm that G4 dendrimers cross the lipid membranes containing PS, which then become more permeable to ions. The obtained results have implications for PAMAM dendrimers as delivery vehicles to cells.

Publiceringsår

2014

Språk

Engelska

Sidor

12892-12906

Publikation/Tidskrift/Serie

The Journal of Physical Chemistry Part B

Volym

118

Issue

45

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Natural Sciences
  • Physical Chemistry
  • Physical Sciences

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1520-5207