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Detection of Helicobacter species in liver and stomach tissues of patients with chronic liver diseases using polymerase chain reaction-denaturing gradient gel electrophoresis and immunohistochemistry

In English

Författare

  • P Stalke
  • Waleed Abu Al-Soud
  • K P Bielawski
  • A Bakowska
  • H Trocha
  • J Stepinski
  • Torkel Wadström

Summary, in English

Objective. Helicobacter DNA has been detected in the hepatobiliary tree of patients with chronic liver diseases (CLD). The presence of H. pylori in the stomach compared with in the liver of the same patients with CLD has not been studied, therefore to the aim of this study was to investigate the presence of Helicobacter DNA and antigens in the liver and stomach of Polish patients with chronic liver diseases using molecular and immunological methods. Material and methods. Gastric mucosa and liver tissue samples and sera were collected from 97 Polish patients with CLD. Anti-H. pylori antibodies were detected by enzyme immunoassay (EIA), and H. pylori-like antigens detected by immunohistochemistry. Helicobacter DNA was detected in stomach and liver samples using a semi-nested Helicobacter genus-specific polymerase chain reaction (PCR) assay, and Helicobacter species identified by denaturing gradient gel electrophoresis (DGGE) and sequencing analysis of amplified PCR products. Results. H. pylori was identified by DGGE and sequence analysis in 60/62 (97%) and 25/25 (100%) of the gastric and liver Helicobacter genus-positive samples, respectively, whereas DNA of H. heilmannii was detected in 2/62 (3%) of the Helicobacter genus-positive gastric samples. H. pylori cagA gene was detected in 23/62 (36%) and 3/25 (12%) gastric and liver tissue samples, respectively. H. pylori-like antigens were detected in 61/97 (63%) gastric mucosa and in 40/97 (41%) liver tissue samples. Conclusions. H. pylori - like organisms appeared to dominate the gastric mucosa and liver tissue of Polish patients with CLD. The prevalence of the cagA gene was higher in stomach compared with liver samples, which suggests a possible role of cagA negative H. pylori - like organisms in CLD. On the other hand, no significant correlation was found between the presence of H. pylori - like DNA and antigens in the liver and liver function tests.

Publiceringsår

2005

Språk

Engelska

Sidor

1032-1041

Publikation/Tidskrift/Serie

Scandinavian Journal of Gastroenterology

Volym

40

Issue

9

Dokumenttyp

Artikel i tidskrift

Förlag

Taylor & Francis

Ämne

  • Gastroenterology and Hepatology

Nyckelord

  • PCR-DGGE
  • Helicobacter pylori
  • DNA sequencing
  • CagA
  • chronic liver diseases
  • 16S rDNA

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1502-7708